Prestained protein ladder western blot (2024)

provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. Certificates of Analysis and Kit Components Lists are located under the Documents tab. Please see the Kit Components List to determine kit components. Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. CLEARLY Stained Protein Ladder is sufficient for 50 lanes. QuantityĬLEARLY Protein Ladder (Unstained) is sufficient for 100 lanes. They remain stable for up to 50 freeze-thaw cycles. These products are stable for 6 months when stored at 4☌.

• Ensure that the ladder is completely thawed before removing an aliquot for loading.
• Pre-stained ladder (Cat.# 3454): Load 10 µl per lane.
• Unstained ladder (Cat.# 3453): Load 5 µl per lane.
• *Reference band (four-fold greater intensity relative to the other bands) SDS-PAGE recommendations
• CLEARLY Stained Protein Ladder (Cat.# 3454A and 3454B).
• *Reference band (four-fold greater intensity relative to the other bands)
• CLEARLY Protein Ladder (Unstained) (Cat.# 3453A and 3453B).
• # 3453A for complete product documentation and resources. In this case, this ladder is sufficient for 100 lanes.Cat. 5 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. This ladder is pre-mixed with loading buffer and ready to load. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators.

  

  This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. When this ladder is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, it is resolved into quite clearly identifiable bands from 10 to 250 kDa. Migration pattern of CLEARLY Stained Protein Ladder (Cat.# 3454A) on an 12% SDS-PAGE gel Migration pattern of CLEARLY Stained Protein Ladder (Cat.# 3454A) on an 12% SDS-PAGE gel.ĬLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins. ＊: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. # 3454A for complete product documentation and resources. ＊: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest.Cat. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting. In this case, this ladder is sufficient for 50 lanes. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. For most researchers, we generally recommend:Ī) ab116027 for proteins between 10 and 180 kDaī) ab116028 for larger proteins up to 245 kDaĬ) ab116029 for smaller proteins down to 3.5 kDaĭ) ab116029 or ab234592 for studying smaller and larger proteins at the same time.CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins. When choosing the right pre-stained protein ladder, the most important choice is based on the size of your protein of interest. Prestained ladders also can use highlight bands of a different color, to make it easier to identify different size bands. Protein ladders are most convenient to use when they are supplied ready to use, with no heating, dilution, or addition of reducing agent required before loading onto the gel. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel. However, an unstained protein ladder can only be visualized following staining with Coomassie or a similar non-specific protein stain. Using an unstained protein ladderis very useful when you need to accurately calculate the size of your protein. calculate the approximate size of your protein by overlaying an image of the membrane with the ladder with the image generated by antibody staining. verify transfer efficiency between gel and membrane (PVDF, nylon, or nitrocellulose) monitor protein separation during SDS-polyacrylamide gel electrophoresis

  

  Using a prestained protein ladder when running a western blot helps when you need to: